Paul's companion, a Gentile of Ephesus (Acts 21:29). Accompanied him on his return from his third missionary journey through Asia to Jerusalem. While Tychicus, his associate, a fellow Asiatic, was left behind on the route (Acts 20:4) Trophimus went forward with Paul. The Jews raised a tumult supposing Paul had introduced Trophimus a Gentile convert into the temple. Paul left Trophimus sick at Miletus just before his own second Roman imprisonment (2 Timothy 4:12; 2 Timothy 4:20).

Trophimus was probably one of the two brethren who with Titus carried the Second Epistle to the Corinthians (2 Corinthians 8:16-24, especially 2 Corinthians 8:22, since 2 Corinthians 8:18 refers to Luke). Trophimus was probably the brother sent before with Titus (2 Corinthians 12:18), and therefore must have been sent from Ephesus; he was moreover an Ephesian. A Gentile like Titus. Connected with Paul in the mission of collecting for the poor in Judaea; he was moreover with Paul on his return from this very visit to Corinth. Tradition makes him beheaded by Nero.